1 Sec DNA Prep from Cultured Cells
The easiest DNA preparation
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Add 100 µL of LazyExtract Solution to 5,000 to 100,000 cells and mix.
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Use the mixture as a PCR template or store it at room temperature.
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Heating process (65ËšC 6 min, 98ËšC 2 min) increases long-term storage stability, but is not necessary if samples are used within one week.
Samples prepared with LazyExtract™ are very stable
Robust PCR bands can be obtained even from DNA samples prepared using LazyExtract under lazy storage conditions.
Human iPSCs, 1 x 10^5 cells/100 µL
Mix → storage
PCR amplicon size: 2004 bp
* Detergent + buffer
Long term sample storage at room temperature
Similar products offer easy PCR sample preparation, but the DNA degrades even at -20ËšC. "LazyExtracted DNA" has the same stability at room temperature and at -20ËšC, and it is far superior to the similar products.
Human iPSCs, 1 x 10^4 cells/100 µL
Mix → heat (65ËšC 6 min, 98ËšC 2 min) → storage (1 month)
PCR amplicon size: 176 bp
PCR after 1-month storage
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